《植物生理学报》 2010, 46(1): 29-32

种皮蜡质与乌拉尔甘草种子抗菌及抗老化能力的关系

张文静, 孙群* , 王建华, 孙宝启

中国农业大学农学与生物技术学院植物遗传育种系, 农业部基因组学与遗传改良重点实验室, 北京市作物遗传改良重点实验室, 北京100193

通信作者：孙群；E-mail: sqcau@126.com；Tel: 010-62732775

摘　要：

本文研究了乌拉尔甘草种子种皮蜡质的厚度与蜡质抑菌效果、蜡质与种子抗老化能力和种子吸水能力的关系。结 果表明: 用浓硫酸浸泡2~3 min可完全除去蜡质, 蜡质的厚度约为30 μm; 接种菌液后, 脱蜡种子在PDA培养基上形成的青霉 和曲霉菌斑的直径显著大于不脱蜡的种子形成的菌斑直径, 但蜡质的体外抗菌能力不明显; 随着老化时间的延长, 脱蜡种 子的含水量增加速度和发芽率下降速度明显快于未脱蜡种子, 且在湿度较高的环境下, 脱蜡种子的平衡水分比未脱蜡种子 显著升高, 说明蜡质在一定程度上可以保护种子不易受外界湿度的影响。

关键词：乌拉尔甘草; 种子; 蜡质; 抗菌; 抗老化

收稿：2009-08-31   修定：2009-11-18

资助：国家科技攻关课题(2001BA701A59)和国家科技支撑计划(2006BA106A15)。

Relation between Surface Wax and the Ability of Antisepsis and Anti-Aging of Licorice (Glycyrrhiza uralensis Fisch.) Seeds

ZHANG Wen-Jing, SUN Qun*, WANG Jian-Hua, SUN Bao-Qi

Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China

Corresponding author: SUN Qun; E-mail: sqcau@126.com; Tel: 010-62732775

Abstract:

The relation between surface wax and its ability of antisepsis, anti-aging and water-content in licorice (Glycyrrhiza uralensis Fisch.) seeds was analyzed. The result showed that the wax thickness of seed surface was about 30 μm, and it could be completely removed by soaked with concentrated sulfuric acid for 2–3 min. After inoculated with mildew, the dewaxed seeds formed bigger blaques on PDA medium than the control seeds, but the antiseptic effect of wax in vitro was not obvious. With aging, the water content of dewaxed seeds rose more quickly than control, with much more quickly declining in germination rate. Under high humidity environment, the equilibrium water content of the dewaxed seeds was significantly higher than the original seeds, indicated that the wax could protect the seeds from intrusion of external humidity.

Key words: licorice (Glycyrrhiza uralensis); seed; wax; antiseptic; anti-aging